{"product_id":"caplugs-240-9000-010-immunostain-moisture-chamber-ps-natural","title":"10–100 µL Immunostain Moisture Chambers, PS, Natural — 240-9000-010 | 3\/Bag | Caplugs® Evergreen","description":"\u003ch2\u003eImmunostain Moisture Chambers, Polystyrene (PS), Natural — 240-9000-010 | 3\/Bag\u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e\u003cth colspan=\"2\"\u003eQuick Reference — 240-9000-010\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eImmunostain Moisture Chamber\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eColor\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eSlide Capacity\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eMultiple standard 25 × 75 mm slides (varies by configuration)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrimary Use\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eIHC, IF, FISH antibody incubation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eAutoclave\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e✓ Compatible (121°C \/ 15 psi)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eSterility\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e3 \/ Bag\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrice\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e$142.48 \/ Bag ($47.4905 \/ chamber)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen is a natural Polystyrene (PS) immunostain moisture chamber for IHC, IF, FISH, ISH, and other slide-based antibody incubation protocols requiring a controlled humid environment to prevent reagent evaporation during incubation. Evaporation of small-volume antibody solutions (10–100 µL) from slide surfaces during primary and secondary antibody incubation causes concentration gradients, uneven staining, edge artifacts, and false-positive signal — particularly in high-sensitivity assays such as phospho-antibody IHC, proximity ligation assays, and multiplex fluorescent panels. The moisture chamber eliminates these artifacts by maintaining a saturated humid environment around slides during incubation at room temperature, 4°C, or 37°C. Natural (translucent) PS allows external visual monitoring of slide moisture and reagent coverage without opening the chamber, reducing the risk of disturbing slides during extended incubations.\u003c\/p\u003e \u003cp\u003eVirgin PS construction delivers autoclave compatibility for decontamination between staining runs, UV decontamination compatibility for clean-bench environments, and broad resistance to standard IHC reagents including PBS, TBS, citrate and EDTA antigen retrieval buffers, hematoxylin, eosin, and DAB chromogen. PS rigidity maintains chamber geometry under full slide loads without flex that could allow the lid seal to open during incubation and compromise humidity. The chamber floor is designed to hold distilled water or damp tissue at a defined depth below the slide surface, establishing consistent humidity without risk of fluid contact with the slide or tissue section.\u003c\/p\u003e \u003cp\u003eAt $142.48 per 3\/Bag — $47.4905 per chamber — the delivers controlled-humidity incubation at a per-chamber cost that supports dedicated allocation per staining run or protocol. Also available: 240-9020-Z10 (PS Black, $142.48\/Bag); 240-9035-N10 (PS Amber, $142.48\/Bag) — enabling matched procurement across all three light-handling specifications from a single vendor.\u003c\/p\u003e \u003ch2\u003eTechnical Specifications — Caplugs® Evergreen \u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eSpecification\u003c\/th\u003e \u003cth\u003eValue\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003ePart Number\u003c\/td\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCaplugs Item #\u003c\/td\u003e \u003ctd\u003e2409000010E1\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eProduct Type\u003c\/td\u003e \u003ctd\u003eImmunostain Moisture Chamber\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMaterial\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eColor\u003c\/td\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSlide Format\u003c\/td\u003e \u003ctd\u003eStandard 25 × 75 mm microscope slides\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eLight Properties\u003c\/td\u003e \u003ctd\u003eTranslucent — visual monitoring without opening\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSterility\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePack Size\u003c\/td\u003e \u003ctd\u003e3 \/ Bag\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eReorder Unit\u003c\/td\u003e \u003ctd\u003eBag of 3\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave\u003c\/td\u003e \u003ctd\u003e✓ Compatible (121°C \/ 15 psi)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eApplication\u003c\/td\u003e \u003ctd\u003eIHC, IF, FISH, ISH, PLA antibody incubation; humid slide staining environments\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBPA \/ Phthalate\u003c\/td\u003e \u003ctd\u003eBPA-free; no phthalate plasticizers\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCountry of Origin\u003c\/td\u003e \u003ctd\u003eMade in U.S.A. (California)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eImmunostaining Workflow Compatibility — \u003c\/h2\u003e \u003cp\u003eThe natural immunostain moisture chamber maintains the humid environment required across the following slide-based immunostaining and in situ hybridization workflows:\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eSpecimen \/ Use\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eImmunohistochemistry (IHC)\u003c\/td\u003e \u003ctd\u003ePrimary antibody incubation, overnight staining\u003c\/td\u003e \u003ctd\u003eHumid environment prevents antibody solution evaporation from slides\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eImmunofluorescence (IF)\u003c\/td\u003e \u003ctd\u003ePrimary and secondary antibody incubation\u003c\/td\u003e \u003ctd\u003ePrevents non-uniform staining from evaporation-driven concentration gradients\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFISH \/ ISH\u003c\/td\u003e \u003ctd\u003eProbe hybridization and washing steps\u003c\/td\u003e \u003ctd\u003eMaintains humidity during extended hybridization incubations\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eH\u0026amp;E and special stains\u003c\/td\u003e \u003ctd\u003eExtended incubation staining protocols\u003c\/td\u003e \u003ctd\u003eLess critical than IHC but useful for high-humidity environments\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCISH \/ chromogenic ISH\u003c\/td\u003e \u003ctd\u003eChromogenic probe incubation\u003c\/td\u003e \u003ctd\u003eSame humidity requirements as FISH for even probe distribution\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eProximity ligation assay (PLA)\u003c\/td\u003e \u003ctd\u003eAntibody and ligation incubation\u003c\/td\u003e \u003ctd\u003ePLA reagent volumes (10–50 µL) particularly vulnerable to evaporation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePhospho-antibody staining\u003c\/td\u003e \u003ctd\u003ePhosphorylation-sensitive IHC panels\u003c\/td\u003e \u003ctd\u003ePrevents evaporation artifacts that can mimic phospho-signal\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMultiplex IHC \/ mIF panels\u003c\/td\u003e \u003ctd\u003eSequential antibody stripping and restaining\u003c\/td\u003e \u003ctd\u003eConsistent humidity across all cycles reduces inter-cycle variability\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003cp\u003e\u003cem\u003eAdd distilled water or damp tissue to the chamber floor to establish humidity before placing slides. Do not flood the floor — capillary wicking onto the slide surface will dilute reagents.\u003c\/em\u003e\u003c\/p\u003e \u003ch2\u003eChemical \u0026amp; Environmental Resistance — Polystyrene (PS)\u003c\/h2\u003e \u003cp\u003ePS provides excellent resistance to aqueous IHC buffers and standard staining reagents. Avoid xylene and acetone — PS is solvent-sensitive.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers (PBS, TBS, citrate)\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eShort-chain alcohols (EtOH, IPA 70%)\u003c\/td\u003e \u003ctd\u003e✓ Good — standard rinse concentration\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDilute acids (\u0026lt;10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eHematoxylin \/ eosin staining\u003c\/td\u003e \u003ctd\u003e✓ Compatible\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDAB chromogen (brief contact)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eXylene\u003c\/td\u003e \u003ctd\u003e✗ Avoid — PS solvent sensitive\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eConcentrated organic solvents\u003c\/td\u003e \u003ctd\u003e✗ Not recommended\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave (121°C \/ 15 psi)\u003c\/td\u003e \u003ctd\u003e✓ Compatible\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eUV decontamination (254 nm)\u003c\/td\u003e \u003ctd\u003e✓ Compatible\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eClinical \u0026amp; Research Laboratory Applications\u003c\/h2\u003e \u003cp\u003eThe moisture chamber serves anatomic pathology and histology laboratories performing IHC panels for tumor diagnostics, biomarker profiling, and companion diagnostic testing; research laboratories running IF, FISH, and ISH protocols on tissue sections and cytology preparations; multiplex IHC and multispectral imaging programs requiring consistent antibody incubation across sequential staining cycles; proximity ligation assay workflows; phospho-protein IHC panels where evaporation-driven concentration errors are particularly impactful; and any pathology or research setting where slide-based antibody incubation is performed outside an automated stainer.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eEvery Caplugs® Evergreen lot is precision-molded or manufactured at our California facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. \u003ca href=\"#request-sample\"\u003eRequest a compatibility sample below\u003c\/a\u003e to validate fit with your workflow before committing to volume.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions — Caplugs® Evergreen \u003c\/h2\u003e \u003ch3\u003eWhy does IHC require a moisture chamber — can't I just cover slides with a coverslip?\u003c\/h3\u003e \u003cp\u003eCoverslips prevent evaporation but create challenges with reagent distribution and antibody kinetics at low volumes. A moisture chamber allows antibody solutions to be applied directly to the slide surface (coverslip-free) at volumes of 50–200 µL, which is the standard manual IHC technique. The chamber maintains the humid environment that prevents evaporation of these micro-volumes during incubation — without the coverslip-related reagent trapping and distribution artifacts that complicate downstream washing steps.\u003c\/p\u003e \u003ch3\u003eWhat is the difference between the Natural, Black, and Amber moisture chambers?\u003c\/h3\u003e \u003cp\u003eAll three are PS moisture chambers with identical dimensions and humidity performance. The difference is light handling: Natural  is translucent — allows visual monitoring of slide moisture. Black (240-9020-Z10) completely excludes ambient light — use for photosensitive fluorescent secondary antibodies, TSA signal amplification, and fluorescent probe-based assays. Amber (240-9035-N10) filters UV wavelengths for partial protection of UV-sensitive antibody conjugates in mixed-protocol labs. Choose based on the photosensitivity requirements of your reagents.\u003c\/p\u003e \u003ch3\u003eCan the moisture chamber be autoclaved between staining runs?\u003c\/h3\u003e \u003cp\u003eYes. The is molded from Polystyrene (PS) and is autoclave-compatible at 121°C \/ 15 psi for decontamination between staining runs. PS is also UV decontamination-compatible for clean-bench environments. Note: PS is sensitive to xylene and acetone — do not use these solvents to clean the chamber.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"Immunostain Moisture Chambers, Polystyrene (PS), Natural \\u2014 240-9000-010 | 3\/Bag | Caplugs\\u00ae Evergreen\",\n  \"description\": \"Caplugs\\u00ae Evergreen 240-9000-010. Immunostain moisture chamber, PS Natural, 3\/Bag. IHC, IF, FISH incubation. BPA-free. USA.\",\n  \"sku\": \"2409000010E1\",\n  \"mpn\": \"240-9000-010\",\n  \"material\": \"Polystyrene (PS)\",\n  \"color\": \"Natural\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Caplugs\\u00ae Evergreen\"\n  },\n  \"manufacturer\": {\n    \"@type\": \"Organization\",\n    \"name\": \"Caplugs\\u00ae Evergreen\",\n    \"address\": {\n      \"@type\": \"PostalAddress\",\n      \"addressCountry\": \"US\",\n      \"addressRegion\": \"CA\"\n    }\n  },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"price\": \"142.48\",\n    \"priceCurrency\": \"USD\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"itemCondition\": \"https:\/\/schema.org\/NewCondition\",\n    \"seller\": {\n      \"@type\": \"Organization\",\n      \"name\": \"LabSupplies\"\n    }\n  },\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack Size\",\n      \"value\": \"3 \/ Bag\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Sterility\",\n      \"value\": \"Non-Sterile\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Country of Origin\",\n      \"value\": \"United States\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"BPA Free\",\n      \"value\": \"Yes\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Why does IHC require a moisture chamber \\u2014 can't I just cover slides with a coverslip?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Coverslips prevent evaporation but create challenges with reagent distribution and antibody kinetics at low volumes. A moisture chamber allows antibody solutions to be applied directly to the slide surface (coverslip-free) at volumes of 50\\u2013200 \\u00b5L, which is the standard manual IHC technique. The chamber maintains the humid environment that prevents evaporation of these micro-volumes during incubation \\u2014 without the coverslip-related reagent trapping and distribution artifacts that complicate downstream washing steps.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the difference between the Natural, Black, and Amber moisture chambers?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"All three are PS moisture chambers with identical dimensions and humidity performance. The difference is light handling: Natural (240-9000-010) is translucent \\u2014 allows visual monitoring of slide moisture. Black (240-9020-Z10) completely excludes ambient light \\u2014 use for photosensitive fluorescent secondary antibodies, TSA signal amplification, and fluorescent probe-based assays. Amber (240-9035-N10) filters UV wavelengths for partial protection of UV-sensitive antibody conjugates in mixed-protocol labs. Choose based on the photosensitivity requirements of your reagents.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Can the 240-9000-010 moisture chamber be autoclaved between staining runs?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. The 240-9000-010 is molded from Polystyrene (PS) and is autoclave-compatible at 121\\u00b0C \/ 15 psi for decontamination between staining runs. PS is also UV decontamination-compatible for clean-bench environments. Note: PS is sensitive to xylene and acetone \\u2014 do not use these solvents to clean the chamber.\"\n      }\n    }\n  ]\n}\n\u003c\/script\u003e","brand":"Caplugs® Evergreen","offers":[{"title":"Default Title","offer_id":48563789103336,"sku":"240-9000-010","price":142.48,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0694\/5361\/1240\/files\/240-9000-010.png?v=1778908682","url":"https:\/\/labsupplies.com\/products\/caplugs-240-9000-010-immunostain-moisture-chamber-ps-natural","provider":"LabSupplies.com","version":"1.0","type":"link"}