{"product_id":"222-8032-01r-microplate","title":"0.32 mL Microplates, Conical Bottom, PS, Natural — 222-8032-01R | 100\/Box | Caplugs® Evergreen","description":"\u003ch2\u003eQuick Reference\u003c\/h2\u003e \u003ctable\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003ePart Number\u003c\/th\u003e \u003ctd\u003e222-8032-01R\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eFormat\u003c\/th\u003e \u003ctd\u003e96-well microplate with matching lid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eWell Bottom\u003c\/th\u003e \u003ctd\u003eRound\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eWell Geometry\u003c\/th\u003e \u003ctd\u003eU-shaped round well bottom — gentle curve for suspension cell culture and mixing\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eWell Volume\u003c\/th\u003e \u003ctd\u003e0.32 mL max per well\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMaterial\u003c\/th\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eColor\u003c\/th\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eLid\u003c\/th\u003e \u003ctd\u003eIncluded — individually packed plate + lid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSterility\u003c\/th\u003e \u003ctd\u003eSterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e100\/Box Individually Packed\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrimary Use\u003c\/th\u003e \u003ctd\u003eSuspension lymphocyte culture, mixed lymphocyte reaction (MLR), ELISpot, NK cell assays\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eOrigin\u003c\/th\u003e \u003ctd\u003eMade in USA\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrice\u003c\/th\u003e \u003ctd\u003e$241.77\/Box ($2.4177\/plate)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen \u003cstrong\u003e222-8032-01R\u003c\/strong\u003e is a \u003cstrong\u003e96-Well Round Bottom PS Sterile Microplate with Lid\u003c\/strong\u003e, 0.32 mL per well, individually packed, 100\/Box. The round (U-shaped) well bottom provides a gentle curved surface suited to suspension cell culture — lymphocytes and other non-adherent cells settle into a loose cluster at the bottom center of the round well, maintaining cell-cell contact for proliferation assays and cytokine stimulation while avoiding the harsh cell pellet concentration of a V-bottom. Round-bottom plates are standard for mixed lymphocyte reactions (MLR), NK cell cytotoxicity assays, ELISpot precursor cultures, and T-cell activation assays.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003ePS cell culture-grade polystyrene\u003c\/strong\u003e: surface-treated for enhanced cell attachment in adherent culture formats, optically clear for plate reader compatibility (400–750 nm), low autofluorescence for fluorescence-based detection. Each plate ships individually wrapped with its matching lid — sterility maintained until the individual wrapper is opened at the BSC. BPA-free, phthalate-free, FDA-compliant, \u003c\/p\u003e \u003cp\u003eProcurement economics: \u003cstrong\u003e$241.77\/Box (100 plates)\u003c\/strong\u003e, \u003cstrong\u003e$2.4177\/plate\u003c\/strong\u003e. 96-well set: \u003cstrong\u003e222-8030-01F\u003c\/strong\u003e (96-Well Flat Bottom 0.37 mL); \u003cstrong\u003e222-8031-01V\u003c\/strong\u003e (96-Well Conical Bottom 0.30 mL); \u003cstrong\u003e222-8033-01C\u003c\/strong\u003e (96-Well Chamfered Bottom 0.35 mL). LabSupplies.com ships from USA inventory, typically same business day.\u003c\/p\u003e \u003ch2\u003eTechnical Specifications\u003c\/h2\u003e \u003ctable\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003ePart Number\u003c\/th\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCaplugs Item #\u003c\/th\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eFormat\u003c\/th\u003e \u003ctd\u003e96-well microplate with matching lid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eWell Bottom\u003c\/th\u003e \u003ctd\u003eRound\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eWell Geometry\u003c\/th\u003e \u003ctd\u003eU-shaped round well bottom — gentle curve for suspension cell culture and mixing\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eWell Volume\u003c\/th\u003e \u003ctd\u003e0.32 mL max per well\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMaterial\u003c\/th\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eColor\u003c\/th\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eLid\u003c\/th\u003e \u003ctd\u003eIncluded — individually packed with plate\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSterility\u003c\/th\u003e \u003ctd\u003eSterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e100\/Box Individually Packed\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrimary Application\u003c\/th\u003e \u003ctd\u003eSuspension lymphocyte culture, mixed lymphocyte reaction (MLR), ELISpot, NK cell assays\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePlate Reader\u003c\/th\u003e \u003ctd\u003eCompatible — SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eBPA \/ Phthalate\u003c\/th\u003e \u003ctd\u003eBPA-Free, Phthalate-Free, Latex-Free\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCountry of Origin\u003c\/th\u003e \u003ctd\u003eUSA \u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrice\u003c\/th\u003e \u003ctd\u003e$241.77\/Box ($2.4177\/plate)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003e96-Well Round Bottom Microplate Applications\u003c\/h2\u003e \u003cp\u003eThe is compatible with the following assay and cell biology workflows.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eSetting \/ Configuration\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003eSuspension lymphocyte culture\u003c\/th\u003e \u003ctd\u003eIncubator, round-bottom 96-well\u003c\/td\u003e \u003ctd\u003eU-bottom: cells cluster gently without harsh pellet formation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMixed lymphocyte reaction (MLR)\u003c\/th\u003e \u003ctd\u003eAllogeneic cell co-culture, 96-well\u003c\/td\u003e \u003ctd\u003eRound bottom: cell-cell contact for proliferation assay\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eNK cell cytotoxicity\u003c\/th\u003e \u003ctd\u003eE:T ratio assay, 96-well round bottom\u003c\/td\u003e \u003ctd\u003eU-bottom: target cell clustering for NK contact\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eELISpot precursor culture\u003c\/th\u003e \u003ctd\u003ePre-stimulation culture, round-bottom\u003c\/td\u003e \u003ctd\u003eSuspension culture for antigen-specific T-cell stimulation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eT-cell activation assay\u003c\/th\u003e \u003ctd\u003eAnti-CD3\/CD28 stimulation, 96-well\u003c\/td\u003e \u003ctd\u003eRound bottom: T-cell clustering for activation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCell co-culture\u003c\/th\u003e \u003ctd\u003eMixed cell population culture\u003c\/td\u003e \u003ctd\u003eU-bottom: cell-cell interaction without selective attachment\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eAntibody production hybridoma\u003c\/th\u003e \u003ctd\u003e96-well round-bottom cloning\u003c\/td\u003e \u003ctd\u003eRound bottom: single-cell HAT selection culture\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eChemical Resistance — Polystyrene (PS) Cell Culture Microplate\u003c\/h2\u003e \u003cp\u003eCompatibility reference for PS at typical assay and cell culture conditions.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers \/ saline\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePS compatible across pH 4–10.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell culture media (RPMI, DMEM)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePS: standard cell culture material.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFormalin (10% NBF)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003eCompatible for cell fixation.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMethanol (\u0026lt;100%)\u003c\/td\u003e \u003ctd\u003eLimited\u003c\/td\u003e \u003ctd\u003eShort-term compatible; avoid prolonged contact.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (\u0026lt;1%)\u003c\/td\u003e \u003ctd\u003eGood\u003c\/td\u003e \u003ctd\u003eCompatible at standard assay concentrations.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDetergents (Tween-20, Triton)\u003c\/td\u003e \u003ctd\u003eGood\u003c\/td\u003e \u003ctd\u003eCompatible at standard assay concentrations.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone \/ chloroform\u003c\/td\u003e \u003ctd\u003eNot Compatible\u003c\/td\u003e \u003ctd\u003eDissolves PS; do not use.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eUV (\u0026lt;320 nm)\u003c\/td\u003e \u003ctd\u003eNot Transparent\u003c\/td\u003e \u003ctd\u003ePS opaque to UV; use UV-transparent plates for UV absorbance.\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eClinical \u0026amp; Research Laboratory Applications\u003c\/h2\u003e \u003cp\u003eCaplugs® Evergreen 96-well round (U-bottom) PS sterile microplates serve immunology, transplant medicine, and cell therapy laboratories performing suspension lymphocyte assays in the 96-well plate format. The mixed lymphocyte reaction (MLR) — used in transplant immunology for donor-recipient HLA compatibility assessment — requires a round-bottom plate where allogeneic lymphocytes co-culture in suspension with cell-to-cell contact maintained by the U-shaped well geometry. NK cell cytotoxicity assays (chromium release, flow cytometry-based), ELISpot precursor stimulation cultures, and antigen-specific T-cell proliferation assays also specify the round-bottom format.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eCaplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.\u003c\/p\u003e \u003cp\u003eLabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can \u003ca href=\"#request-sample\"\u003erequest a written quote or speak with a product specialist\u003c\/a\u003e directly from our sales engineering team.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e \u003ch3\u003eAre the 96-well microplates sterile?\u003c\/h3\u003e \u003cp\u003eYes. Sterile — each plate individually packed with its matching lid, 100\/Box.\u003c\/p\u003e \u003ch3\u003eWhat is the complete 96-well microplate bottom geometry set from Caplugs Evergreen?\u003c\/h3\u003e \u003cp\u003eFour bottom geometries: Flat (222-8030-01F, 0.37 mL), Conical V-bottom (222-8031-01V, 0.30 mL), Round U-bottom (, 0.32 mL), Chamfered (222-8033-01C, 0.35 mL). All are sterile PS with matching lids, individually packed 100\/Box.\u003c\/p\u003e \u003ch3\u003eWhen should I use a round-bottom  vs a V-bottom (222-8031-01V)?\u003c\/h3\u003e \u003cp\u003eRound U-bottom for suspension cell biology where gentle cell clustering is needed — MLR, NK cytotoxicity, T-cell activation, ELISpot precursor culture. V-bottom for agglutination assays and tight pellet concentration. The key difference is cell viability after the incubation: round-bottom cells are less stressed and more viable for downstream assays.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\/\", \"@type\": \"Product\", \"name\": \"Caplugs® Evergreen 222-8032-01R | 96-Well Microplates with Lids, Round Bottom, 0.32 mL\/Well, Sterile — PS, Natural, Individually Packed\", \"sku\": \"222803201RE1\", \"mpn\": \"222-8032-01R\", \"brand\": {\"@type\": \"Brand\", \"name\": \"Caplugs® Evergreen\"}, \"manufacturer\": {\"@type\": \"Organization\", \"name\": \"Caplugs® Evergreen\", \"address\": \"USA\"}, \"material\": \"Polystyrene (PS)\", \"color\": \"Natural\", \"image\": \"https:\/\/prod-cp-cdn-images.azureedge.net\/catalog\/92300\/CB133AD1-CB0C-4B1A-9B7F-8FD51EDA3E77\/96%20Well%20Plate%20PS%20with%20Lid.png\", \"description\": \"Caplugs® Evergreen 222-8032-01R: 96-Well Round Bottom Microplates with Lids, 0.32 mL\/Well, Sterile, PS, Natural, Individually Packed, 100\/Box. MADE IN.\", \"additionalProperty\": [{\"@type\": \"PropertyValue\", \"name\": \"Wells\", \"value\": \"96\"}, {\"@type\": \"PropertyValue\", \"name\": \"Well Bottom\", \"value\": \"Round\"}, {\"@type\": \"PropertyValue\", \"name\": \"Well Volume\", \"value\": \"0.32 mL\"}, {\"@type\": \"PropertyValue\", \"name\": \"Pack\", \"value\": \"100\/Box IP\"}, {\"@type\": \"PropertyValue\", \"name\": \"Sterility\", \"value\": \"Sterile\"}, {\"@type\": \"PropertyValue\", \"name\": \"Origin\", \"value\": \"USA\"}], \"offers\": {\"@type\": \"Offer\", \"priceCurrency\": \"USD\", \"price\": \"241.77\", \"availability\": \"https:\/\/schema.org\/InStock\", \"url\": \"https:\/\/labsupplies.com\/products\/222-8032-01r-microplate\"}}\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\/\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"Are the 222-8032-01R 96-well microplates sterile?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Sterile — each plate individually packed with its matching lid, 100\/Box.\"}}, {\"@type\": \"Question\", \"name\": \"What is the complete 96-well microplate bottom geometry set from Caplugs Evergreen?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Four bottom geometries: Flat (222-8030-01F, 0.37 mL), Conical V-bottom (222-8031-01V, 0.30 mL), Round U-bottom (222-8032-01R, 0.32 mL), Chamfered (222-8033-01C, 0.35 mL). All are sterile PS with matching lids, individually packed 100\/Box.\"}}, {\"@type\": \"Question\", \"name\": \"When should I use a round-bottom (222-8032-01R) vs a V-bottom (222-8031-01V)?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Round U-bottom for suspension cell biology where gentle cell clustering is needed — MLR, NK cytotoxicity, T-cell activation, ELISpot precursor culture. V-bottom for agglutination assays and tight pellet concentration. The key difference is cell viability after the incubation: round-bottom cells are less stressed and more viable for downstream assays.\"}}]}\u003c\/script\u003e","brand":"Caplugs® Evergreen","offers":[{"title":"Default Title","offer_id":48567931896040,"sku":"222803201RE1","price":241.77,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0694\/5361\/1240\/files\/96_20Well_20Plate_20PS_20with_20Lid_c9cba812-10d1-4411-8df4-034a7c12d8cd.png?v=1779074503","url":"https:\/\/labsupplies.com\/products\/222-8032-01r-microplate","provider":"LabSupplies.com","version":"1.0","type":"link"}