{"product_id":"222-3529-g8d-sterile-freestanding-centrifuge","title":"25–30 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3529-G8D | 20\/Tray | Caplugs® Evergreen","description":"\u003ch2\u003eQuick Reference\u003c\/h2\u003e \u003ctable\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003ePart Number\u003c\/th\u003e \u003ctd\u003e222-3529-G8D\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCapacity\u003c\/th\u003e \u003ctd\u003e30 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMaterial\u003c\/th\u003e \u003ctd\u003ePolypropylene Copolymer (PPC)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eColor\u003c\/th\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eBase\u003c\/th\u003e \u003ctd\u003eFreestanding (stands without rack)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCap\u003c\/th\u003e \u003ctd\u003eGreen screw cap — pre-assembled in tray\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e20\/Tray Assembled\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSterility\u003c\/th\u003e \u003ctd\u003eSterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eOrigin\u003c\/th\u003e \u003ctd\u003eMade in USA\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrice\u003c\/th\u003e \u003ctd\u003e$519.55\/Tray ($25.9775\/tube)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen \u003cstrong\u003e222-3529-G8D\u003c\/strong\u003e is a \u003cstrong\u003e30 mL Sterile PPC Freestanding Centrifuge Tube with Green Cap\u003c\/strong\u003e, 20\/Tray Assembled, sterile. The freestanding base allows the tube to stand upright without a rack during bench processing — eliminating rack dependency in the BSC for high-throughput cell culture workflows. The 30 mL sterile format serves large-volume cell culture harvest, suspension culture scale-up, and any sterile processing workflow requiring more than the standard 15 mL conical tube capacity. The 20\/Tray Assembled format presents pre-assembled green-capped tubes in an open sterile rack tray for direct BSC pick-and-use without tube assembly steps.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003ePolypropylene Copolymer (PPC)\u003c\/strong\u003e: full ACN\/MeOH organic solvent compatibility, improved cold-temperature flexibility vs PS, optical clarity for visual pellet and gradient monitoring. BPA-free, phthalate-free, FDA-compliant, \u003c\/p\u003e \u003cp\u003eProcurement economics: \u003cstrong\u003e$519.55\/Tray\u003c\/strong\u003e (20\/Tray), \u003cstrong\u003e$25.9775\/tube\u003c\/strong\u003e. Sibling: \u003cstrong\u003e222-3530-G80\u003c\/strong\u003e (30 mL PPC Green Cap 10\/Bag Unassembled). LabSupplies.com ships from USA inventory, typically same business day.\u003c\/p\u003e \u003ch2\u003eTechnical Specifications\u003c\/h2\u003e \u003ctable\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003ePart Number\u003c\/th\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCaplugs Item #\u003c\/th\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCapacity\u003c\/th\u003e \u003ctd\u003e30 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eGeometry\u003c\/th\u003e \u003ctd\u003eConical bottom, freestanding base\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMaterial\u003c\/th\u003e \u003ctd\u003ePolypropylene Copolymer (PPC)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eColor\u003c\/th\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCap\u003c\/th\u003e \u003ctd\u003eGreen screw cap — pre-assembled in tray\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSterility\u003c\/th\u003e \u003ctd\u003eSterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e20\/Tray Assembled\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003ctd\u003eSterile 30 mL cell culture, large-volume harvest, freestanding bench use\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eBPA \/ Phthalate\u003c\/th\u003e \u003ctd\u003eBPA-Free, Phthalate-Free, Latex-Free\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCountry of Origin\u003c\/th\u003e \u003ctd\u003eUSA \u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrice\u003c\/th\u003e \u003ctd\u003e$519.55\/Tray ($25.9775\/tube)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003e30 mL Sterile PPC Freestanding Centrifuge Tube Applications\u003c\/h2\u003e \u003cp\u003eThe is compatible with the following sterile large-volume workflows.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eSetting \/ Configuration\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003eCell culture harvest (30 mL)\u003c\/th\u003e \u003ctd\u003eSuspension culture, centrifuge, BSC\u003c\/td\u003e \u003ctd\u003eSterile; freestanding for BSC rack-free handling\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eBench centrifuge\u003c\/th\u003e \u003ctd\u003eStandard rotor, 30 mL adapter, up to 3000×g\u003c\/td\u003e \u003ctd\u003ePPC withstands centrifuge forces; verify adapter\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eT-cell expansion\u003c\/th\u003e \u003ctd\u003eLarge-volume T-cell culture, 25–30 mL\u003c\/td\u003e \u003ctd\u003eSterile PPC; green cap re-seals after sampling\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eDensity gradient centrifugation\u003c\/th\u003e \u003ctd\u003eFicoll\/Percoll, 30 mL sterile tube\u003c\/td\u003e \u003ctd\u003eSterile conical tip; PPC clarity for interface monitoring\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eBSC workflow\u003c\/th\u003e \u003ctd\u003eOpen tray — direct pick-and-use\u003c\/td\u003e \u003ctd\u003eFreestanding: upright without rack throughout\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCold storage\u003c\/th\u003e \u003ctd\u003e4°C \/ —20°C, freestanding tube\u003c\/td\u003e \u003ctd\u003ePPC: cold-temperature flexibility\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSPE eluate collection (sterile)\u003c\/th\u003e \u003ctd\u003eUnder sterile SPE manifold, 30 mL\u003c\/td\u003e \u003ctd\u003ePPC: ACN\/MeOH compatible for sterile extraction\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eChemical Resistance — Polypropylene Copolymer (PPC)\u003c\/h2\u003e \u003cp\u003eCompatibility reference for PPC at typical laboratory conditions.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers \/ saline\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePPC inert across pH 2–12.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMethanol \/ acetonitrile (\u0026lt;100%)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003eFull organic solvent compatibility.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFormalin (10% NBF)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003eCompatible for fixation protocols.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eTransport media (UTM, VTM)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePPC compatible with viral transport media.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell culture media\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePPC: standard sterile cell culture material.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (\u0026lt;10%)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePPC compatible with cryoprotectant concentrations.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDensity gradient media\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003eFicoll\/Percoll compatible.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDichloromethane \/ chloroform\u003c\/td\u003e \u003ctd\u003eNot Compatible\u003c\/td\u003e \u003ctd\u003eCauses cracking; do not use.\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eClinical \u0026amp; Research Laboratory Applications\u003c\/h2\u003e \u003cp\u003eCaplugs® Evergreen 30 mL sterile PPC freestanding centrifuge tubes with green caps serve cell therapy, cell biology, and clinical microbiology laboratories requiring sterile large-volume centrifuge tube capacity above the standard 15 mL conical. Cell therapy manufacturing uses sterile 30 mL freestanding tubes for cell harvest, washing, and concentration steps in T-cell expansion and CAR-T manufacturing workflows where the 15 mL tube is insufficient for the cell culture volume and the freestanding base allows tube handling in the BSC without a rack. PPC is specified over PS for the same reasons as other PPC sterile tubes — methanol-based intracellular staining post-harvest and cold-storage of stained cells before flow cytometry analysis. The 20\/Tray Assembled format provides pre-assembled tubes in an open sterile tray for direct BSC use without assembly — the preferred format for cell therapy GMP environments where assembly steps add process steps to the batch record.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eCaplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.\u003c\/p\u003e \u003cp\u003eLabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can \u003ca href=\"#request-sample\"\u003erequest a written quote or speak with a product specialist\u003c\/a\u003e directly from our sales engineering team.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e \u003ch3\u003eAre the 30 mL freestanding centrifuge tubes sterile?\u003c\/h3\u003e \u003cp\u003eYes. Sterile — packed in sterile tray or sterile bag as specified.\u003c\/p\u003e \u003ch3\u003eWhat is the difference between 20\/Tray Assembled  and 10\/Bag Unassembled (222-3530-G80)?\u003c\/h3\u003e \u003cp\u003e20\/Tray Assembled: pre-assembled green cap + tube in open sterile tray — direct BSC pick-and-use, no assembly step, preferred for cell therapy GMP and high-throughput BSC workflows. Higher per-tube cost. 10\/Bag Unassembled: tube and cap in same bag, assembled at bench — lower per-tube cost for standard research labs.\u003c\/p\u003e \u003ch3\u003eWhy use a sterile 30 mL tube versus the non-sterile 220-3530-080?\u003c\/h3\u003e \u003cp\u003eThe sterile 222-3529\/3530 series is required when the tube will directly contact cells in culture, receive a sterile specimen for downstream sterile processing, or be used in a GMP cell therapy workflow where sterility documentation is required per batch record. The non-sterile 220-3530-080 is suited to analytical sample prep, SPE eluate collection, and bench chemistry where sterility is not required.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\/\", \"@type\": \"Product\", \"name\": \"Caplugs® Evergreen 222-3529-G8D | 30 mL Freestanding Centrifuge Tubes with Green Caps, Sterile — PPC, Natural, 20\/Tray Assembled\", \"sku\": \"2223529G8DE1\", \"mpn\": \"222-3529-G8D\", \"brand\": {\"@type\": \"Brand\", \"name\": \"Caplugs® Evergreen\"}, \"manufacturer\": {\"@type\": \"Organization\", \"name\": \"Caplugs® Evergreen\", \"address\": \"USA\"}, \"material\": \"Polypropylene Copolymer (PPC)\", \"color\": \"Natural\", \"image\": \"https:\/\/prod-cp-cdn-images.azureedge.net\/catalog\/92300\/83974D1C-8579-4A6E-B2D8-EF5C2EAE548C\/30%20mL%20Cent%20Tube.png\", \"description\": \"Caplugs® Evergreen 222-3529-G8D: 30 mL Freestanding Centrifuge Tubes with Green Caps, Sterile, PPC, Natural, 20\/Tray Assembled. 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The non-sterile 220-3530-080 is suited to analytical sample prep, SPE eluate collection, and bench chemistry where sterility is not required.\"}}]}\u003c\/script\u003e","brand":"Caplugs® Evergreen","offers":[{"title":"Default Title","offer_id":48567661199592,"sku":"2223529G8DE1","price":519.55,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0694\/5361\/1240\/files\/30_20mL_20Cent_20Tube_7f626737-a631-4b87-9222-6741163ef6b5.png?v=1779053224","url":"https:\/\/labsupplies.com\/products\/222-3529-g8d-sterile-freestanding-centrifuge","provider":"LabSupplies.com","version":"1.0","type":"link"}