{"product_id":"222-2012-010-sterile-round-bottom-test-tube","title":"50–100 µL Test Tubes, Round Bottom, PS, Natural, Beckman\/BD Compatible — 222-2012-010 | 25\/Bag | Caplugs® Evergreen","description":"\u003ch2\u003eQuick Reference\u003c\/h2\u003e \u003ctable\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003ePart Number\u003c\/th\u003e \u003ctd\u003e222-2012-010\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCapacity\u003c\/th\u003e \u003ctd\u003e5.0 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eDimensions\u003c\/th\u003e \u003ctd\u003e12 × 75 mm\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMaterial\u003c\/th\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eColor\u003c\/th\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCap \/ Closure\u003c\/th\u003e \u003ctd\u003eNone — open-top tube; use separate stopper or cap\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSterility\u003c\/th\u003e \u003ctd\u003eSterile — individually or bag sterile-packed\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e25\/Bag\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eKey Use\u003c\/th\u003e \u003ctd\u003eFlow cytometry (FACS), serology, blood bank tube testing\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eOrigin\u003c\/th\u003e \u003ctd\u003eMade in USA\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrice\u003c\/th\u003e \u003ctd\u003e$89.84\/Bag ($3.5935\/tube)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen \u003cstrong\u003e222-2012-010\u003c\/strong\u003e is a \u003cstrong\u003e5.0 mL Round Bottom PS Sterile Test Tube\u003c\/strong\u003e, 12 × 75 mm, open top, 25\/Bag, sterile. The 5.0 mL 12×75 mm round-bottom PS tube is the industry-standard flow cytometry tube — the universal format for FACS\/cell sorting sample preparation, immunophenotyping, and cell biology assays. This is the same tube geometry used in virtually every commercial flow cytometer sample holder worldwide. Open-top format allows pipette access from above for sample loading and reagent dispensing before capping or reading.\u003c\/p\u003e \u003cp\u003ePS provides the optical clarity required for direct visual reading of agglutination reactions in tube serology — the test result is observed as a button, partial agglutinate, or dispersed cell pattern against the clear tube wall. PS also provides the low autofluorescence background important for fluorescence-based flow cytometry detection.\u003c\/p\u003e \u003cp\u003eProcurement economics: \u003cstrong\u003e$89.84\/Bag\u003c\/strong\u003e, \u003cstrong\u003e$3.5935\/tube\u003c\/strong\u003e. Sterile 222-series round-bottom test tube siblings: \u003cstrong\u003e222-2036-050\u003c\/strong\u003e (5.0 mL 12×75 mm Two-Position Snap Cap 25\/Bag Unassembled); \u003cstrong\u003e222-2036-05I\u003c\/strong\u003e (5.0 mL 12×75 mm Two-Position Snap Cap Individually Packed); \u003cstrong\u003e222-2003-010\u003c\/strong\u003e (4.0 mL 10×75 mm Open Top 25\/Bag). LabSupplies.com ships from USA inventory, typically same business day.\u003c\/p\u003e \u003ch2\u003eTechnical Specifications\u003c\/h2\u003e \u003ctable\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003ePart Number\u003c\/th\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCaplugs Item #\u003c\/th\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCapacity\u003c\/th\u003e \u003ctd\u003e5.0 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eDimensions\u003c\/th\u003e \u003ctd\u003e12 × 75 mm\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eGeometry\u003c\/th\u003e \u003ctd\u003eRound bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eMaterial\u003c\/th\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eColor\u003c\/th\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCap\u003c\/th\u003e \u003ctd\u003eNone (open top); use compatible stopper or closure\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSterility\u003c\/th\u003e \u003ctd\u003eSterile — individually or bag sterile-packed\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e25\/Bag \u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003ctd\u003eFlow cytometry (FACS), cell sorting, immunophenotyping; standard 12×75 mm format\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eBPA \/ Phthalate\u003c\/th\u003e \u003ctd\u003eBPA-Free, Phthalate-Free, Latex-Free\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCountry of Origin\u003c\/th\u003e \u003ctd\u003eUSA \u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrice\u003c\/th\u003e \u003ctd\u003e$89.84\/Bag ($3.5935\/tube)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003e5.0 mL 12×75 mm Sterile PS Round Bottom Tube Applications\u003c\/h2\u003e \u003cp\u003eThe is the standard flow cytometry and serology tube. Compatible with the following platforms and protocols.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication \/ Instrument\u003c\/th\u003e \u003cth\u003eConfiguration\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003cth\u003eFlow cytometer (FACS)\u003c\/th\u003e \u003ctd\u003eStandard 12×75 mm tube holder (BD FACSCanto, LSR, Attune, CytoFLEX, etc.)\u003c\/td\u003e \u003ctd\u003eIndustry-standard 12×75 mm format — fits all major flow cytometer tube holders\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCell sorter\u003c\/th\u003e \u003ctd\u003e12×75 mm sample tube position\u003c\/td\u003e \u003ctd\u003eOpen-top or snap-cap for sample loading; cap removed for cell sorter nozzle\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eTube serology (blood bank)\u003c\/th\u003e \u003ctd\u003eManual tube agglutination, centrifuge\u003c\/td\u003e \u003ctd\u003ePS clarity for visual agglutination reading\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eImmunophenotyping\u003c\/th\u003e \u003ctd\u003eSurface marker staining, lyse\/wash protocol\u003c\/td\u003e \u003ctd\u003e5 mL capacity for standard staining volumes (50–100 µL sample + 200 µL reagents)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCell culture \/ stimulation\u003c\/th\u003e \u003ctd\u003eShort-term culture in incubator (snap-cap)\u003c\/td\u003e \u003ctd\u003eTwo-position snap cap: vented for CO2 exchange, closed for transport\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eIntracellular staining\u003c\/th\u003e \u003ctd\u003eFixation\/permeabilization protocol\u003c\/td\u003e \u003ctd\u003ePS: compatible with methanol, saponin-based protocols\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCentrifugation\u003c\/th\u003e \u003ctd\u003eCell pellet, up to 600×g wash steps\u003c\/td\u003e \u003ctd\u003eRound bottom: uniform cell pellet; sterile for downstream culture\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eChemical Resistance — Polystyrene (PS)\u003c\/h2\u003e \u003cp\u003eCompatibility reference for PS at typical laboratory conditions.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers \/ saline\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePS compatible across pH 4–10 for standard biological assays.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSerum \/ plasma \/ whole blood\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003eStandard clinical and blood bank specimen matrix.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFormalin (10% NBF)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePS compatible with standard fixative for flow cytometry fixation.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMethanol (\u0026lt;100%)\u003c\/td\u003e \u003ctd\u003eLimited\u003c\/td\u003e \u003ctd\u003eShort-term compatible for intracellular staining fixation steps.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSaponin \/ detergent buffers\u003c\/td\u003e \u003ctd\u003eGood\u003c\/td\u003e \u003ctd\u003ePS compatible with saponin-based permeabilization buffers.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone \/ chloroform\u003c\/td\u003e \u003ctd\u003eNot Compatible\u003c\/td\u003e \u003ctd\u003eDissolves PS; do not use.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (\u0026lt;1%)\u003c\/td\u003e \u003ctd\u003eGood\u003c\/td\u003e \u003ctd\u003eCompatible at concentrations used in cell culture and cryopreservation.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFlow cytometry reagents (FITC, PE, APC, etc.)\u003c\/td\u003e \u003ctd\u003eExcellent\u003c\/td\u003e \u003ctd\u003ePS: low autofluorescence in visible wavelength range for cytometry.\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eClinical \u0026amp; Research Laboratory Applications\u003c\/h2\u003e \u003cp\u003eCaplugs® Evergreen 5.0 mL 12×75 mm sterile PS round-bottom test tubes are the universal format for flow cytometry and immunophenotyping in clinical immunology, hematology, oncology, and research settings. The 12×75 mm round-bottom tube is the standard sample tube for virtually all commercial flow cytometers — BD FACSCanto, LSRFortessa, Attune NxT, Beckman Coulter CytoFLEX, Miltenyi MACSQuant, and others all use the 12×75 mm tube holder as the primary sample input format. Clinical immunophenotyping laboratories performing CD4\/CD8 T-cell counting, lymphocyte subset analysis for AIDS and lymphoma, and leukemia\/lymphoma immunophenotyping by flow cytometry standardize on the sterile 5.0 mL PS round-bottom tube for staining and data acquisition. The open-top format allows pipette access from above for antibody cocktail addition, lysis buffer dispensing, and wash steps before analysis.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eCaplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.\u003c\/p\u003e \u003cp\u003eLabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can \u003ca href=\"#request-sample\"\u003erequest a written quote or speak with a product specialist\u003c\/a\u003e directly from our sales engineering team.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e \u003ch3\u003eAre the test tubes sterile?\u003c\/h3\u003e \u003cp\u003eYes. Sterile — individually or bag sterile-packed as specified. Maintain sterility until the bag or individual wrapper is opened.\u003c\/p\u003e \u003ch3\u003eIs the 5.0 mL 12 × 75 mm tube compatible with my flow cytometer?\u003c\/h3\u003e \u003cp\u003eYes. The 12×75 mm round-bottom tube is the industry-standard flow cytometer sample tube format used by BD (FACSCanto, LSRFortessa, Aria), Beckman Coulter (CytoFLEX, Gallios), Miltenyi (MACSQuant), Thermo (Attune), and virtually all other major flow cytometer platforms that accept standard tube input. Verify your specific instrument's tube holder specification before switching suppliers.\u003c\/p\u003e \u003ch3\u003eWhat is the difference between sterile (222-series) and non-sterile (214-series) round-bottom PS tubes?\u003c\/h3\u003e \u003cp\u003eThe 222-series tubes are sterile-packed — required for applications involving direct patient specimen contact, cell culture, or any downstream sterile processing step. The 214-series tubes are non-sterile and suited to general diagnostic serology, mixing, and sample prep where sterility is not required. The tube material (PS) and geometry are equivalent across series.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\/\", \"@type\": \"Product\", \"name\": \"Caplugs® Evergreen 222-2012-010 | 5.0 mL Round Bottom Test Tubes, 12 × 75 mm, Sterile — PS, Natural, 25\/Bag\", \"sku\": \"2222012010E1\", \"mpn\": \"222-2012-010\", \"brand\": {\"@type\": \"Brand\", \"name\": \"Caplugs® Evergreen\"}, \"manufacturer\": {\"@type\": \"Organization\", \"name\": \"Caplugs® Evergreen\", \"address\": \"USA\"}, \"material\": \"Polystyrene (PS)\", \"color\": \"Natural\", \"image\": \"https:\/\/prod-cp-cdn-images.azureedge.net\/catalog\/92300\/9093F9E9-0629-4E5F-B21B-39C8A1BB4B87\/214-2012-010_02.jpg\", \"description\": \"Caplugs® Evergreen 222-2012-010: 5.0 mL Round Bottom Test Tubes, 12 × 75 mm, Sterile, PS, 25\/Bag. 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