{"product_id":"208-3045-020-surety-column-fine-filter","title":"3.5–5.0 mL Adapters, LDPE, Natural — 208-3045-020 | 1000\/Bag | Caplugs® Evergreen","description":"\u003ch2\u003e3.5–5.0 mL Surety® SPE Columns with Fine Filter (15–45 µm) — LDPE, Natural, Standard 2.3″ Style (208-3045-020)\u003c\/h2\u003e\n\u003cp\u003eThe Caplugs® Evergreen Surety® Standard column (208-3045-020) is a 3.5–5.0 mL capacity solid-phase extraction column featuring a 15–45 µm fine-filter frit, molded in natural Low-Density Polyethylene (LDPE), and supplied non-sterile in bags of 1000. The Standard 2.3″ body accommodates higher sample-load SPE workflows matched to 5 mL or 15 mL collection vessels, while the fine-filter pore size delivers high-clarity filtrate suited for LC-MS\/MS injection preparation, confirmatory reference methods, dissolution testing, and pharmaceutical QC applications where residual particulates drive analytical column fouling and injection carryover.\u003c\/p\u003e\n\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePart Number (MPN)\u003c\/td\u003e\n\u003ctd\u003e208-3045-020\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Type\u003c\/td\u003e\n\u003ctd\u003eSurety® SPE Column with Fine Filter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorking Capacity\u003c\/td\u003e\n\u003ctd\u003e3.5–5.0 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColumn Style\u003c\/td\u003e\n\u003ctd\u003eStandard 2.3″\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFilter Pore Size\u003c\/td\u003e\n\u003ctd\u003e15–45 µm (Fine Filter)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMaterial\u003c\/td\u003e\n\u003ctd\u003eLow-Density Polyethylene (LDPE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eNatural\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility\u003c\/td\u003e\n\u003ctd\u003eNon-Sterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e1000\/Bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBPA \/ Phthalate \/ Latex\u003c\/td\u003e\n\u003ctd\u003eBPA-Free, Phthalate-Free, Latex-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd\u003eUSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLC-MS\/MS sample preparation: filtration of protein precipitation filtrate to remove residual particulates before injection\u003c\/li\u003e\n\u003cli\u003eConfirmatory reference methods: GC-MS and LC-UV post-SPE cleanup requiring high-clarity filtrate to protect analytical columns\u003c\/li\u003e\n\u003cli\u003ePharmaceutical QC: dissolution filtration compatible with aqueous dissolution media per USP Apparatus I\/II requirements\u003c\/li\u003e\n\u003cli\u003eBioanalytical and pharmacokinetic studies: plasma and urine extract cleanup for higher-volume sample loads\u003c\/li\u003e\n\u003cli\u003eGeneral SPE workflows using 5 mL or 15 mL collection tube formats on vacuum manifolds or benchtop centrifuges\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCompatibility\u003c\/h2\u003e\n\u003cp\u003eThe Standard 2.3″ Surety® column is sized for 5 mL and 15 mL collection tube SPE workflows and can be operated on vacuum manifolds from Waters, Phenomenex, Supelco, and Agilent in tube-format configurations (verify tube adapter fit for your specific manifold model). It is also compatible with bench microcentrifuge protocols up to 3000×g. The defining advantage of the LDPE construction is pressure-assisted manual elution: the flexible column walls allow the analyst to apply gentle finger compression to accelerate filtrate flow without a centrifuge or vacuum manifold, making these columns highly practical for low-throughput or resource-limited settings. For labs requiring smaller working volumes, Caplugs® Evergreen offers the Surety® Mini in the 1.5–2.4 mL range (208-3040-020, 1.5″ body) and the Surety® Midi in the 2.4–3.5 mL range (208-3042-020, 1.9″ body) for complete size-matching across sample loads.\u003c\/p\u003e\n\n\u003ch2\u003eChemical Resistance\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eReagent \/ Condition\u003c\/th\u003e\n\u003cth\u003eCompatibility\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAqueous buffers and saline\u003c\/td\u003e\n\u003ctd\u003eExcellent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMethanol\u003c\/td\u003e\n\u003ctd\u003eGood — standard SPE wash\/elution use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcetonitrile\u003c\/td\u003e\n\u003ctd\u003eGood — standard SPE wash\/elution use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDilute acids and bases (SPE modifiers)\u003c\/td\u003e\n\u003ctd\u003eGood — short-term contact\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChlorinated solvents (e.g., chloroform, DCM)\u003c\/td\u003e\n\u003ctd\u003eNot recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAromatic solvents (e.g., toluene, xylene)\u003c\/td\u003e\n\u003ctd\u003eNot recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eQuality \u0026amp; Procurement\u003c\/h2\u003e\n\u003cp\u003eMade in USA, these Surety® columns are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free LDPE resin at Caplugs® Evergreen's U.S. manufacturing facility. LabSupplies.com is an authorized dealer and ships from USA inventory, typically same business day for stocking items, with volume pricing available for institutional purchasers, bioanalytical labs, and grant-funded research groups ordering multiple bags.\u003c\/p\u003e\n\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eDo these columns require a vacuum manifold or centrifuge?\u003c\/strong\u003e\u003cbr\u003eNo — the flexible LDPE body allows manual pressure-assisted elution by gentle finger compression, so a centrifuge or vacuum manifold is not required. They are equally compatible with vacuum manifolds and benchtop centrifuge protocols for higher-throughput workflows.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhat pore size is the filter frit, and why does it matter for LC-MS\/MS work?\u003c\/strong\u003e\u003cbr\u003eThe filter frit is 15–45 µm (fine filter). This pore range removes residual protein precipitation debris and other fine particulates from sample extracts before injection, protecting analytical columns from fouling and reducing injection carryover — a common source of variability in quantitative LC-MS\/MS assays.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAre these columns sterile?\u003c\/strong\u003e\u003cbr\u003eNo — they are supplied non-sterile in 1000\/Bag packaging, which is standard for sample preparation consumables used in analytical chemistry and SPE workflows.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eHow does the Standard 2.3″ Surety® column compare to the Mini and Midi sizes?\u003c\/strong\u003e\u003cbr\u003eThe Standard 2.3″ body (208-3045-020) handles 3.5–5.0 mL working volumes and is matched to 5 mL or 15 mL collection tubes for higher sample loads. The Surety® Mini (208-3040-020) covers 1.5–2.4 mL and the Surety® Midi (208-3042-020) covers 2.4–3.5 mL, allowing labs to select the right column size for their specific sample volume without switching suppliers or platforms.\u003c\/p\u003e\n\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\":\"https:\/\/schema.org\/\",\"@type\":\"Product\",\"name\":\"3.5–5.0 mL Surety® SPE Columns with Fine Filter (15–45 µm), Standard 2.3″ Style — LDPE, Natural, 1000\/Bag\",\"sku\":\"2083045020E1\",\"mpn\":\"208-3045-020\",\"brand\":{\"@type\":\"Brand\",\"name\":\"Caplugs® Evergreen\"},\"material\":\"Low-Density Polyethylene (LDPE)\",\"color\":\"Natural\",\"offers\":{\"@type\":\"Offer\",\"priceCurrency\":\"USD\",\"price\":\"153.72\",\"availability\":\"https:\/\/schema.org\/InStock\"}}\u003c\/script\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\":\"https:\/\/schema.org\/\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"Do these columns require a vacuum manifold or centrifuge?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"No — the flexible LDPE body allows manual pressure-assisted elution by gentle finger compression, so a centrifuge or vacuum manifold is not required. 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